Dynein Antagonizes Eg5 by Crosslinking and Sliding Antiparallel Microtubules
نویسندگان
چکیده
منابع مشابه
Dynein Antagonizes Eg5 by Crosslinking and Sliding Antiparallel Microtubules
Mitotic spindle assembly requires the combined activity of various molecular motor proteins, including Eg5 and dynein. Together, these motors generate antagonistic forces during mammalian bipolar spindle assembly; what remains unknown, however, is how these motors are functionally coordinated such that antagonism is possible. Given that Eg5 generates an outward force by crosslinking and sliding...
متن کاملThe functional antagonism between Eg5 and dynein in spindle bipolarization is not compatible with a simple push-pull model.
During cell division, the molecular motor Eg5 crosslinks overlapping antiparallel microtubules and pushes them apart to separate mitotic spindle poles. Dynein has been proposed as a direct antagonist of Eg5 at the spindle equator, pulling on antiparallel microtubules and favoring spindle collapse. Some of the experiments supporting this hypothesis relied on endpoint quantifications of spindle p...
متن کاملSpindle Fusion Requires Dynein-Mediated Sliding of Oppositely Oriented Microtubules
BACKGROUND Bipolar spindle assembly is critical for achieving accurate segregation of chromosomes. In the absence of centrosomes, meiotic spindles achieve bipolarity by a combination of chromosome-initiated microtubule nucleation and stabilization and motor-driven organization of microtubules. Once assembled, the spindle structure is maintained on a relatively long time scale despite the high t...
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Ciliary doublet microtubules produced by sliding disintegration in 20 muM MgATP2-reassociate in the presence of exogenous 30S dynein and 6 mM MgSO4. The doublets form overlapping arrays, held together by dynein cross-bridges. Dynein arms on both A and B subfibers serve as unambiguous markers of microtubule polarity within the arrays. Doublets reassociate via dynein cross-bridges in both paralle...
متن کاملDynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly.
Bipolar spindle assembly critically depends on the microtubule plus-end-directed motor Eg5 that binds antiparallel microtubules and slides them in opposite directions. As such, Eg5 can produce the necessary outward force within the spindle that drives centrosome separation and inhibition of this antiparallel sliding activity results in the formation of monopolar spindles. Here, we show that upo...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2009
ISSN: 0960-9822
DOI: 10.1016/j.cub.2009.09.025